This record currently stands at 279 and is held by Hybu Pobl Ifanc and the Newport Gwent Dragons, so in the week before England take on Wales at Twickenham, Harlequins will aim to beat their record at The Stoop.
Alongside Harlequins and England players, coaches and staff, supporters will come together to have fun and raise money for this very worthwhile cause.
All money raised will go towards Sport Relief which supports essential projects at home and abroad and changes lives forever.
As one of the UK's biggest fundraising events, Sport Relief brings the entire nation together to get active, raise cash and change lives.
Comic Relief spends the money raised by the public to give extremely vulnerable and disadvantaged people in the UK and throughout the world a helping hand to turn their lives around.
Commenting on the world records, Harlequins Customer Experience Manager Jessica Webb said: "This is the perfect opportunity for all supporters to have some fun and raise money for a very worthwhile cause.
"We are sure it will be very entertaining and would encourage everyone to come to The Stoop on Saturday, join in and help us raise huge amounts of money for this fantastic charity."
For more information and to get involved in this unique event please visit www.quins.co.uk.