England Head Coach Stuart Lancaster said: "This match has become an established fixture in the season and is hugely important for us ahead of the tour. We are holding a short conditioning camp this week for those players whose season finished on the 5th May and already there is a real buzz about this Barbarians match and what lies ahead after that.
"We will obviously be without the Leicester and Harlequins players - and best of luck to both those teams in the Aviva Premiership final - but this is a great opportunity for the rest of the squad, as well as Freddie, Jamie, Richard and Haydn, to put a marker down before we leave for the tour.
"We are looking forward to going back to Twickenham and if the fantastic support that we got against Wales and Ireland is repeated it will be a great occasion."
Mouritz Botha (Saracens)
Alex Corbisiero (London Irish)
Paul Doran Jones (Northampton Saints)
Phil Dowson (Northampton Saints)
Carl Fearns (Bath Rugby)
Jamie Gibson (London Irish)
Dylan Hartley (Northampton Saints)
Tom Johnson (Exeter Chiefs)
Joe Launchbury (London Wasps)
Lee Mears (Bath Rugby)
Ben Morgan (Scarlets)
Matt Mullan (Worcester Warriors)
Tom Palmer (Stade Francais)
Matt Stevens (Saracens)
Chris Ashton (Northampton Saints)
Brad Barritt (Saracens)
Freddie Burns (Gloucester Rugby)
Lee Dickson (Northampton Saints)
Owen Farrell (Saracens)
Ben Foden (Northampton Saints)
Alex Goode (Saracens)
Charlie Hodgson (Saracens)
Jonathan Joseph (London Irish)
David Strettle (Saracens)
Haydn Thomas (Exeter Chiefs)
Christian Wade (London Wasps)
Richard Wigglesworth (Saracens)
Backs: Nacewa (Leinster), Muliaina (NTT Docomo), Balshaw (Biarritz), Heymans (Bayonne), Williams (Ospreys), Sackey (Stade Francais), Tagicakibau (London Irish), Fuimaono-Sapolu (Gloucester), Tindall (Gloucester), Traille (Biarritz), Contepomi (Stade Francais), Donald (Bath), Jones (Scarlets), Lawson (Gloucester), Fillol (Stade Francais).
Forwards: Brits (Saracens), August (Biarritz), Smit (Saracens), De Malmanche (Stade Francais), Afoa (Ulster), Jones (Ospreys), Botha (Munster), Tialata (Bayonne), Thion (Biarritz), O'Driscoll (Munster), Van Zyl (Stade Francais), Taele (Biarritz), Lakafia (Biarritz), Burban (Stade Francais), Qera (Gloucester), Beattie (Glasgow), Wannenburg (Ulster), Leguizamon (Lyon).
Tickets for the Killik Cup - England v Barbarians on 27th May 2012 are now available, with tickets from £25 for adults and £10 for children. With discounts for groups of 10 or more. Visit rfu.com/tickets to buy now. Booking fees apply and tickets are subject to availability