16,000 tickets will be reserved for the supporters of the two finalists, but the rest will be available for general sale on Thursday, with the best quality tickets normally the first to go.
The Guinness Premiership Final takes place on Saturday 16th May 2009 at Twickenham Stadium and will decide the champions of England.
It will also be the last chance to see some of England's best players before they depart on the British and Irish Lions' tour to South Africa.
Premier Rugby's Head of Marketing and Promotions Jon Duckworth said "Last season we radically changed our marketing approach and that played a major role in the match selling out.
"We have agreed with all twelve clubs that 8,000 tickets will be reserved for each finalist should they make the Guinness Premiership Final, but I would encourage die-hard supporters to purchase their tickets early. Our aim is to sell the final out for a second consecutive year."
Tickets are priced at £45, £35 and £25 for Adults and £10 for Juniors. Tickets can be purchased through Ticketmaster via www.ticketmaster.co.uk or 0844 847 2492.