The scrum half has been replaced by Dan White (Leeds Carnegie) for the game at Worcester on Friday, February 6 (8pm).
"Dave had been enjoying a superb season and we desperately disappointed he's suffered such a setback. Everyone in the camp wishes him all the best for a speedy recovery," said coach Mark Mapletoft
"We hope he's back in action before the end of the season and able to challenge for a place in the squad for the IRB Junior World Championship."
The 22 includes 10 players capped last season, when England completed a Grand Slam in the Six Nations tournament before reaching the final of the IRB Junior World Championship.
England Under 20 squad
Forwards: Bob Baker (London Wasps); Calum Clark (Leeds Carnegie, captain); James Clark (London Irish)
James Gaskell (Sale Sharks); Jamie George (Saracens); Graham Kitchener (Worcester Warriors); Shaun Knight (Gloucester Rugby); Courtney Lawes (Northampton Saints); Ben Moon (Exeter Chiefs); Josh Ovens (Bath Rugby)
Dan Williams (Gloucester Rugby); Chris York (Harlequins)
Backs: Rory Clegg (Newcastle Falcons); Luke Eves (Bristol Rugby); Will Hurrell (Leicester Tigers); Rob Miller (Newcastle Falcons); Charlie Sharples (Gloucester Rugby); Seb Stegmann (Harlequins); Greig Tonks (Leicester Tigers); Henry Trinder (Gloucester Rugby); Dan White (Leeds Carnegie); Ben Youngs (Leicester Tigers)
Tickets are available for international rugby at the home of Worcester Warriors on Friday! Call 01905 454183 today or visit www.warriors.co.uk